The trick is to use the select region BB. You select the border and calculate the area of that border.
Then use calculate properties to get the correct units [µm].
Stack Processing (fixed, string) : Individual Planes (, string) Flatfield Correction (fixed, string) : None (, string) Stack Processing (fixed, string) : Individual Planes (, string) Flatfield Correction (fixed, string) : None (, string) Channel (fixed, string) : DPC (, menu) Select channel for cell detection ROI (fixed, string) : None (, menu) Select population for ROI Method (fixed, string) : B (, menu) Select method for cell detection Common Threshold (fixed, string) : 0.4 (default, numeric) Controls common threshold calculation. Adjust by the tuning illustration Initial Mask. Area (fixed, string) : > (fixed, string) 100 (default, numeric) µm²(fixed, string) Minimum allowed area of cell. All objects with area less than the limit are discarded as artifacts. Split Factor (fixed, string) : 7.0 (default, numeric) The Split Factor influence on splitting of clump cells to the individual objects. Individual Threshold (fixed, string) : 0.4 (default, numeric) The parameter adjusts the object borders, i.e. the detected cells could appear larger or smaller. Contrast (fixed, string) : > (fixed, string) 0.1 (default, numeric) Defines the minimum allowed contrast of cell. All objects with contrast less than the limit are discarded as artifacts. Adjust by the tuning illustration Low Contrast Cells. Output Population (fixed, string) : Cells (, string) Name of the output population Channel (fixed, string) : DPC (, menu) Select channel for cell detection ROI (fixed, string) : None (, menu) Select population for ROI Output Population (fixed, string) : Cells (, string) Name of the output population Population (fixed, string) : Cells (, menu) Select population Region (fixed, string) : Cell (, menu) Select region by which a new region should be created. Method (fixed, string) : Standard (, menu) Select method by which a new region should be created Border (fixed, string) Turn the border calculation ON/OFF Output Regions (fixed, string) : Cell (, string) Common prefix for region names Population (fixed, string) : Cells (, menu) Select population Region (fixed, string) : Cell (, menu) Select region by which a new region should be created. Output Regions (fixed, string) : Cell (, string) Common prefix for region names Population (fixed, string) : Cells (, menu) Select population Region (fixed, string) : Cell Border (, menu) Select region which should be characterized. Method (fixed, string) : Standard (, menu) Select a method Area (fixed, string) Turn the area calculation ON/OFFUnit for area property Output Properties (fixed, string) : Cell Border (, string) Common prefix for the property names Population (fixed, string) : Cells (, menu) Select population Region (fixed, string) : Cell Border (, menu) Select region which should be characterized. Output Properties (fixed, string) : Cell Border (, string) Common prefix for the property names Population (fixed, string) : Cells (, menu) Select population, for which a custom defined property should be calculated. Method (fixed, string) : By Formula (, menu) Select a method Formula (fixed, string) : A (, string) Calculate a new property with formula in style 100*(A-B)/C and thereupon specify below the corresponding variables A, B etc. Please use only single characters (A, B, ..,Y). Variable A (fixed, string) : Cell Border Area [µm²] (, menu) Select a property to which the variable A should correspond to Output Property (fixed, string) : Perimeter [µm] (, string) Name of the calculated property Population (fixed, string) : Cells (, menu) Select population, for which a custom defined property should be calculated. Output Property (fixed, string) : Perimeter [µm] (, string) Name of the calculated property
Analysis Sequence "[New Analysis]"
Analysis Sequence "[New Analysis]"
Input Image
Input Image
Input
Find Cells
Method
Find Cells
Input
Output
Select Region
Method
Select Region
Input
Output
Calculate Morphology Properties
Method
Calculate Morphology Properties
Input
Output
Calculate Properties
Method
Calculate Properties
Input
Output
Define Results
Define Results
Input
Acapella version: 4.1.2.118496. Timestamp: 2016-10-06 10:12:51 +0200.