The trick is to use the select region BB. You select the border and calculate the area of that border.
Then use calculate properties to get the correct units [µm].
| Analysis Sequence "[New Analysis]" |
Analysis Sequence "[New Analysis]"
| Input Image |
Stack Processing (fixed, string) : Individual Planes (, string)
Flatfield Correction (fixed, string) : None (, string)
|
Input Image
Input
Stack Processing (fixed, string) : Individual Planes (, string)
Flatfield Correction (fixed, string) : None (, string)
| Find Cells |
Channel (fixed, string) : DPC (, menu) Select channel for cell detection ROI (fixed, string) : None (, menu) Select population for ROI |
MethodMethod (fixed, string) : B (, menu) Select method for cell detection Common Threshold (fixed, string) : 0.4 (default, numeric) Controls common threshold calculation. Adjust by the tuning illustration Initial Mask. Area (fixed, string) : > (fixed, string) 100 (default, numeric) µm²(fixed, string) Minimum allowed area of cell. All objects with area less than the limit are discarded as artifacts. Split Factor (fixed, string) : 7.0 (default, numeric) The Split Factor influence on splitting of clump cells to the individual objects. Individual Threshold (fixed, string) : 0.4 (default, numeric) The parameter adjusts the object borders, i.e. the detected cells could appear larger or smaller. Contrast (fixed, string) : > (fixed, string) 0.1 (default, numeric) Defines the minimum allowed contrast of cell. All objects with contrast less than the limit are discarded as artifacts. Adjust by the tuning illustration Low Contrast Cells. |
Output Population (fixed, string) : Cells (, string) Name of the output population |
Find Cells
Input
Channel (fixed, string) : DPC (, menu)
Select channel for cell detection
ROI (fixed, string) : None (, menu)
Select population for ROI
Output
Output Population (fixed, string) : Cells (, string)
Name of the output population
| Select Region |
Population (fixed, string) : Cells (, menu) Select population Region (fixed, string) : Cell (, menu) Select region by which a new region should be created. |
MethodMethod (fixed, string) : Standard (, menu) Select method by which a new region should be created Border (fixed, string) Turn the border calculation ON/OFF |
Output Regions (fixed, string) : Cell (, string) Common prefix for region names |
Select Region
Input
Population (fixed, string) : Cells (, menu)
Select population
Region (fixed, string) : Cell (, menu)
Select region by which a new region should be created.
Output
Output Regions (fixed, string) : Cell (, string)
Common prefix for region names
| Calculate Morphology Properties |
Population (fixed, string) : Cells (, menu) Select population Region (fixed, string) : Cell Border (, menu) Select region which should be characterized. |
MethodMethod (fixed, string) : Standard (, menu) Select a method Area (fixed, string) Turn the area calculation ON/OFFUnit for area property |
Output Properties (fixed, string) : Cell Border (, string) Common prefix for the property names |
Calculate Morphology Properties
Input
Population (fixed, string) : Cells (, menu)
Select population
Region (fixed, string) : Cell Border (, menu)
Select region which should be characterized.
Output
Output Properties (fixed, string) : Cell Border (, string)
Common prefix for the property names
| Calculate Properties |
Population (fixed, string) : Cells (, menu) Select population, for which a custom defined property should be calculated. |
MethodMethod (fixed, string) : By Formula (, menu) Select a method Formula (fixed, string) : A (, string) Calculate a new property with formula in style 100*(A-B)/C and thereupon specify below the corresponding variables A, B etc. Please use only single characters (A, B, ..,Y). Variable A (fixed, string) : Cell Border Area [µm²] (, menu) Select a property to which the variable A should correspond to |
Output Property (fixed, string) : Perimeter [µm] (, string) Name of the calculated property |
Calculate Properties
Input
Population (fixed, string) : Cells (, menu)
Select population, for which a custom defined property should be calculated.
Output
Output Property (fixed, string) : Perimeter [µm] (, string)
Name of the calculated property
| Define Results |
Define Results
Input
| Acapella version: 4.1.2.118496. Timestamp: 2016-10-06 10:12:51 +0200. |